Name: Dianne DeCamp
Title: Research Associate Professor
Office: SB 051
Dianne DeCamp arrived at The University of Montana in July 2007 as a Research Associate Professor. She earned a Ph.D. in Biochemistry at the University of Delaware in 1988 under the supervision of Professor Roberta Colman. She completed postdoctoral training in pharmaceutical chemistry and molecular biology under the guidance of Professor Charles Craik at the University of California, San Francisco, in 1993. Her first faculty position was Assistant Professor in the Department of Pathology at Duke University Medical Center from 1993-1995. Afterwards, she held the position of Research Assistant Professor in the Department of Pharmacology at University of Texas Southwestern Medical Center, from 1995 until coming to UM.
Our lab is interested in studying the structure and function of integral membrane proteins, which typically reside and function in a cell’s plasma membrane. Transmembrane proteins, including receptors, are capable of transducing responses from the outside of the cell to the interior through ligand binding and structural rearrangement. They are involved in many aspects of cell homeostasis, including signal transduction, ion and pH regulation and cellular communications, as well as cancer and disease states. Biochemical techniques and X-ray crystallography are used to elucidate ligand binding and the interactions between membrane proteins in complexes. We use eukaryotic and bacterial heterologous expression systems in order to obtain sufficient quantities of proteins for study, and maintain a core facility for insect cell expression. Current projects involve the study of the b2 adrenergic receptor in complex with the G protein heterotrimer, the complex of Junin virus glycoprotein 2 and SSP, and Excitatory Amino Acid Transporter 3 (EAAT3).
Rebres, R.A., Moon, C., DeCamp, D., Lin, K.-M., Fraser, I.D., Milne, S.B., Roach, T.I.A., Brown, H. A. and Seaman, W.E. (2010) Clostridium difficile Toxin B differentially affects GPCR-stimulated Ca2+ responses in macrophages: independent roles for Rho and PLA2. J. Leukoc. Biol. 87: in press. PMC Journal – in Process.
Callender, H.L., Horn, M.A., DeCamp, D.L., Sternweis, P.C., and Brown, H.A. (2009) Modeling species-Specific Diacylglycerol Dynamics in the RAW 264.7 Macrophage. Journal of Theoretical Biology, in press. Callender, H.L, Horn, M.A., DeCamp, D.L., Sternweis, P.C. and Brown, H. A. (2010) Modeling species-specific diacylglycerol dynamics in the RAW 264.7 macrophage. J. Theor. Biol. 262: 679-690.
Roach T.I., Rebres R.A., Fraser I.D., DeCamp D.L., Lin K.-M., Sternweis P.C., Simon M.I., and Seaman W.E. (2008) Signaling and cross-talk by C5a and UDP in macrophages selectively use PLCbeta3 to regulate intracellular free calcium. J Biol Chem. 283:17351-61. PMCID: PMC2427365.
Jiang L.I., Collins J., Davis R. , Lin K.-M., DeCamp D., Roach T., Hsueh R., Rebres R.A., Ross E.M., Taussig R., Fraser I., and Sternweis P.C. (2007) Use of a cAMP BRET sensor to characterize a novel regulation of cAMP by the sphingosine-1-phosphate/G13 pathway. J. Biol. Chem. 282: 10576-84.
Milne S.B., Ivanova P.T., DeCamp D., Hsueh R.C., and Brown H.A. (2005) A targeted mass spectrometric analysis of phosphatidylinositol phosphate species. J. Lipid Res. 46(8): 1796-1802.
Sambrano G.R., Chandy G., Choi S., Decamp D., Hsueh R., Lin K.M., Mock D., O'Rourke N., Roach T., Shu H., Sinkovits B., Verghese M., Bourne H. (2002) Unravelling the signal-transduction network in B lymphocytes. Nature 420(6916), 708-10.
Zhou G., Li H., DeCamp D., Chen S., Shu H., Gong Y., Flaig M., Gillespie J.W., Hu N., Taylor P.R., Emmert-Buck M.R., Liotta L.A., Petricoin E.F. 3rd, Zhao Y. (2002) 2D differential in-gel electrophoresis for the identification of esophageal cell cancer-specific protein markers. Mol. Cell. Proteomics 1(2), 117-24.
Xiao T., DeCamp D.L., Sprang S.R. (2000) Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer. Protein Sci. 9(10),1889-97.
DeCamp D.L., Thompson T.M., de Sauvage F., and Lerner M.R. (2000) Smoothened Activates G?i-Mediated Signaling in Frog Melanophores. J. Biol. Chem. 275(34), 26322-7.
Vash B., Phung N., Zein S., and DeCamp D. (1998) Three Complement-Type Repeats of the Low Density Lipoprotein Receptor-related Protein Define a Common Binding Site for RAP, PAI-1 and Lactoferrin. BLOOD 92, 3277-3285.
Howard G.C., Yamaguchi Y., Misra U.K., Gawdi G., Nelsen A., DeCamp D.L., and Pizzo S.V. (1996) Selective Mutations in Cloned and Expressed a-Macroglobulin Receptor Binding Fragment Alter Binding to Either the alpha-2-Macroglobulin Signaling Receptor or the Low Density Lipoprotein Receptor-related Protein/alpha-2-Macroglobulin Receptor. J. Biol. Chem. 271, 14105-14111.
Howard G.C., Misra U.K., DeCamp D.L., and Pizzo S.V. (1996) Altered Interaction of Cis-dichlorodiammineplatinum (II)-modified alpha-2-Macroglobulin (?2M) with the Low Density Lipoprotein Receptor-related Protein/alpha-2M Receptor but Not the Signaling Receptor. J. Clin. Invest. 97, 1193-1203.
DeCamp D.L., Ogden R.C., Kuntz I.D., and Craik C.S. (1996) Chapter 17: Site-Directed Drug Design. In Protein Engineering: Principles and Practice (J.L. Cleland and C.S. Craik, Eds.), Wiley-Liss, Inc., New York, 467-505.